Cost effective cultivation and biomass production of green microalga desmodesmus subspicatus mb. 23 in npk fertilizer medium

Jasmin Kaippilliparambil Abdulsamad, Saramma Aikkarakunnath Varghese, Jabir Thajudeen

Cost effective cultivation and biomass production of green microalga desmodesmus subspicatus mb. 23 in npk fertilizer medium

Číslo: 3/2019/2020
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2019/20.9.3.599-604

Klíčová slova: Biofuel, Biomass production, Desmodesmus subspicatus, Carotenoids, NPK fertilizer

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Anotace: Microalgal biomass has emerged as a promising alternative to replace plant-based biofuel feedstock due to its higher lipid productivity.

But cultivation of microalgae in expensive analytical grade culture media is a major obstacle in feasible algal biofuel production. Hence
the present investigation was carried out to find an alternative, low-cost culture medium for the increased biomass yield and biochemical
production of microalga, Desmodesmus subspicatus MB. 23. The strain was cultivated in different concentrations of 19: 19: 19 NPK
media and checked for the algal biomass production and biochemical accumulation. Maximum algal cell density (5290 ×104 cells/ ml),
biomass yield and productivity (2.72 g/L, 60.87 mg/L/d) was attained in 2 g/ L NPK fertilizer medium, whereas 1g/L medium exhibited
increased chlorophyll production (chl-a, 4.7 mg/g d wt, chl-b 1.7 mg/g d wt). High level of carotenoid accumulation (4.6 mg/g d wt) as
well as lipid accumulation (29.5%) was found in 0.5g/L fertilizer media and Fatty acid methyl ester analysis of the strain showed
abundant production of C16 and C18 fatty acids. Findings of the current study proved that, lower concentration of 19:19:19 NPK
fertilizer can be used for the enhanced production of green microalgae. Other than cost effective biofuel production, NPK fertilizer
grown D. subspicatus can also be used for the production of a wide range of metabolites such as food and feed additives,
pharmaceuticals and cosmetics.