Anotace:
Copper has found to be one in every of the main trace components among of all found within the human body and additionally co-factor of quite three hundred class enzymes, plays a very important role in maintaining cellular processes which are crucial as well as stress, desoxyribonucleic acid replication, repairing of DNA, progression of cell cycle and programmed cell death. Thus, it has found to be very evident that Associate in Nursing alteration in Copper levels in willcer cells can cause a harmful result. analysis has shown that low concentration of Copper in cells ends up in the initiation and progression of cancer and high concentration of Copper shows harmful effects. Copper-mediated super molecular activity situation and aerophilous stress through reactive oxygen species (ROS) might also be the probable cause of this cytotoxic result. CuO contains a neutral hydroxyl radical connected to its surface, that plays a very important role in the changes of behaviour of the surface . Our aim is to indicate that the result of Copper oxide and silicon coated copper oxide on totally different microbes and cancer cells. Characterization of Cu nanoparticles have been done by using different analyzing techniques i.e. UV-Vis Spectroscopy, DLS (Dynamic Light Scattering) and SEM (Scanning Electron Microscope). The effect of the Cu nanoparticles on microbes has been measured by the cup disk method where as the effect on cancer cell line (HeLa) (Human Cervical Cancer Cell Line) has been measured by Fluorescence Anisotropy, MTT assay, Reactive Oxygen Species (ROS). The effect on different enzymatic action has also been measured.