Restriction polymorphism of mal d 1 allergen promotor in apple varieties

Anotace: Apples are the most consumed fruit in the European Union with the exception of citrus fruits and apple consumption is part of a healthy and balanced diet. Although apples are recommended to consume each day, they are one of the fruits that can trigger an allergic reaction. The most important apple allergen in Central and Northern Europe is Mald 1. The aim of the study was to analyse natural restriction pattern variability of the part of Ypr 10 gene promotor in four apple varieties. All the analysed varieties resulted in different cleavage profiles for Ase I and Spe I when compared to the virtual cleavage profile of the genomic sequence stored in the public nucleotide database.