Anotace:
Black chokeberry (Aronia melanocarpa L.) attracts scientific interest due to its high content of bioactive compounds with beneficial effects on human health. Presented work aimed to determine the total polyphenol content (TPC), total anthocyanin content (TAC), and antioxidant activity by methods ABTS, DPPH, FRAP of black chokeberry (Aronia melanocarpa L.) variety Nero, from 4 localities (Holovousy, Bašnice, Choteč, Veľký Lapáš), fresh, and conserved (with addition of sucrose and subsequent freezing for 3 months). TPC determined by spectrophotometry using the Folin-Ciocalteau reagent were in the range 8646-13213 mg GAE.kg-1 (fresh chokeberry) and 5068-7047 mg GAE.kg-1 (preserved chokeberry). The TAC values, determined spectrophotometrically by lowering the pH of the extract, were in the range 489-875 mg.kg-1 (fresh chokeberry) and 620-1026 mg.kg-1 (preserved chokeberry). The AA values (ABTS, DPPH, FRAP) were 2.35-2.97; 1.79-2.18; 2.01-2.67 mmol TE.kg-1 (fresh chokeberry) and 2.79-3.45; 2.15-2.57; 2.42-2.60 mmol TE.kg-1 (preserved chokeberry). The results obtained show that the location of cultivation has a significant influence on the chemical parameters monitored. The addition of sucrose and freezing resulted in lower TPC and higher TAC in all samples, while it had no clear effect on the AA levels determined by all three methods.