The effect of a single dose of selenium with vitamin E on oxidative stress and fertilization indicators of ram sperm over the course of spermatogenesis

Anotace: The aim of the experiment was to determine the effect of a single subcutaneous administration of selenium (Se) + vitamin E on the ejaculate volume, sperm count and viability, level of apoptosis and oxidative stress (OS) and Se concentration in ejaculates and blood of rams with respect to the time course of the spermatogenic cycle. The experimental group (EG; n = 6) was treated with a single injection of Selevit at a dose of 5 ml per animal (11 mg of sodium selenite and 125 mg of vitamin E/per animal). The control group (CG; n = 4) was treated in the same way, only with saline. Samples of blood and ejaculates were collected from each ram prior to application of Se and on the 1st, 14th, 26th, 38th, 50th and 62nd day after injection. Results showed that the Se concentration in the blood of EG was significantly higher only 24 h after application. Selenium concentration in the ejaculates of the EG was higher during the whole duration of the experiment (62 days), but significantly so only until day 14. The level of OS was significantly reduced on day 1, 14, and 62 after application of Selevit. There were no significant differences in the other analysed indicators. The results showed that one subcutaneous injection of Selevit had a positive effect on Se concentration and OS level in ejaculates, but was not sufficient to improve other monitored sperm quality indicators.