Exploration of antibacterial and antiproliferative secondary metabolites from marine bacillus

Chandini S. Syed, Mantri Sairam, Amrutha V. Audipudi

Exploration of antibacterial and antiproliferative secondary metabolites from marine bacillus

Číslo: 3/2019/2020
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2019/20.9.3.628-633

Klíčová slova: Antibacterial, antiproliferative, A-549 cell line, Bacillus flexus AVSC4, GC-MS analysis, HT-29 cell line

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Anotace: Antiproliferative secondary metabolites producing bacterial strain AVSC4 isolated from marine sediments was identified as Bacillus

flexus based on 16S rRNA gene sequence analysis. Under the strategy of liquid- liquid extraction, the crude extract was obtained
showed significant antibacterial activity against different clinical pathogens. 0.5% methionine and 0.4% NaCl act as inducers for
maximizing the growth and antibacterial activity of strain at pH 7 and 40°C. Heptadecanoic acid and methyl hexadecanoic acid were
identified as major and dominant secondary metabolites by GC-MS analysis and also showed significant antiproliferative activity
against HT-29 (Human colorectal adenocarcinoma) and A-549 (Lung Cancer) with IC50 value 93.4 µg/ml and 50.04 µg/ml.