Evaluation of probiotic candidate in combinationwith tuber extracts from corallocarpus epigaeus (arn.)Cl. Forassessment of in vitro alpha amylase inhibition activity

Anotace: Probiotics are living microbial adjunct which has a beneficial effect on host. Plant extracts such as those from Corralocarpus epigaeus often shows antimicrobial effect. Thus, there is always confusion about the using of herbal medicine or plant extractsinco-administration with probiotics supplement. The aim of the study was to show the synergistic action of probiotic bacteria in combination with plant extract from Coralocarpus epigaeus on alpha amylase inhibition activity. Alpha amylase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycemia via control of starch breakdown. The probiotic bacterial culture in combination with different solvents for plant extraction viz. hexane, chloroform, ethyl acetate and methanol showing higher alpha amylase inhibitory activity compare to organic plant extract. The present study shows that the control having zone of diameter(3cm) and methanolic plant extract (2cm) and with Lactobacillus (0.9cm) which isconsideredgood % inhibition (70%) compare to all organic solvent. While, the methanolic plant extract (2cm) and Bifidobacterium (1cm) also showed moderate % inhibition (66.66%) compare to all organic solvent. The results indicate that combination of plant extracts with probiotic cultures showing highest percentage of alpha amylase inhibition. This study revealed that synergistic actionis probiotic and plant extracts can be used to control post prandial hyperglycemia.