Beneficial effects of rosmarinus officinalis and thymus numidicus on key enzymes of carbohydrate metabolism in alloxan-induced diabetic rats

Anotace: The aim of this study is to evaluate the protective effects of the ethyl acetate fraction of Rosmarinus officinalis (RO-EA) and Thymus numidicus (TN-EA) plant extracts on carbohydrate metabolism enzymes during diabetes mellitus. To achieve this objective, two experiments were carried out. The first of which was evaluating the hypoglycemic and the anti-hyperglycemic effects on normal rats overloaded with glucose (4 mg/kg b.w), with the use of two doses (150 and 300 mg/kg b.w), for each plant in the presence of negative control (vehicle 0.5 ml/100 g b.w), and positive control (5 mg of glibenclamide /kg b.w). The second experiment aims at studying the dose-dependent effect of the two plants’ extracts on carbohydrate metabolism in Wistar rats rendered diabetic by intraperitoneal injection of Alloxan at (120 mg/kg b.w); 54 male rats of the Wistar strain were divided into 09 groups with 06 rats per group. 03 of the groups have normal rats receiving vehicle (0.5 ml/100 g bw), TN-EA (300 mg/kg b.w), RO-EA (300 mg/kg b.w), respectively. The 06 remaining groups were composed of diabetic rats receiving vehicle (0.5 ml/b.w), TN-EA (150 and 300 mg/kg b.w), RO-EA (150 and 300 mg/kg b.w) Glibenclamide (5 mg/kg b.w), respectively. The process lasted for 21 days. Then we proceeded to the assays. Zobrazit více »