Evaluation of the metrological parameters of methods for quantitative determination of active microbial strains of genera pseudomonas and bacillus in the air of biotechnological production zone

Anotace: Provision of safety and sanitary-hygienic control of biotechnological processes poses a special medical-biological challenge requiring elaboration and metrological assessment of quantitative methods, which determine concentrations of microorganisms-producers in air of manufacturing zone in compliance with the criteria of the International Organization for Standardization. The results of experimental modeling of microbial sprays comprising bacteria of genera Bacillus and Pseudomonas in air of the production zone as the risk factor affecting health of bioindustry employees have enabled to calculate metrological parameters of methods to control microorganisms-constituents of monocomponent and bicomponent biological preparations. Such metrological parameters (standard deviation of repeatability, standard deviation of intermediate precision, expanded uncertainty) match the requirements set to the measurement methods at the accepted confidential probability level p=95 % and ensure proper control of biotechnological processes.