Vinayaka B. Shet, K. G. Rakshith, Nisha J. Shetty, Vishwas C. Shetty, Asha Siddik, Louella Concepta Goveas, C. Vaman Rao, P. Ujwal
The application of response surface methodology for the optimization of autoclave assisted hcl hydrolysis of an agro residue cocoa pod shells for releasing reduced sugars
Číslo: 3/2019/2020
Periodikum: Journal of Microbiology, Biotechnology and Food Sciences
DOI: 10.15414/jmbfs.2019/20.9.3.548-551
Klíčová slova: Acid hydrolysis, Cocoa pod shell, Optimization, Reducing sugar
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the cocoa pod shells to release the reducing sugars has been explored. Hydrochloric acid hydrolysis assisted with autoclave was
adopted to obtain reducing sugars. The one parameter at a time approach was implimented to screen the significant parameters
influencing the hydrolysis among weight of cocoa pod shells, concentration of HCl and duration of autoclave. Parameters such as
concentration of HCl and duration of autoclave were found to be significant. These parameters were chosen for the central composite
design comprising of five levels using response surface methodology to optimize the hydrolysis process. At optimized condition of
54.15 minutes of autoclave treatment and 4.41% of HCl, 21.11 g/L of maximum reducing sugar was released. A second order
polynomial equation was generated having a good fit with R2
of 0.84.