Anotace:
The aim of this work was to determine the changes of redox indicators such as reactive oxygen metabolites (dROMs), plasma antioxidant test (PAT) and the oxidative stress index (OSI) in dairy cows at different stages of lactation using a diagnostic equipment which is suitable for in vivo oxidative stress (OS) monitoring procedures. In total, 628 dairy cows were examined in the pre-parturient period (days in milk [DIM]: -21 to -1 day; n = 117), in the calving and maternity period (DIM: 0 to 7; n = 137), in the early lactation period (DIM: 8 to 30; n = 139), and the in the peak lactation (DIM: 31 to 150; n = 235). The dROMs and OSI values were significantly different (P < 0.05) when comparing the 1st and 2nd+ lactation cows in each group. The highest mean value of dROMs was detected at the calving and maternity stage in 1st lactation cows (141 ± 25 U. Carr) and the lowest (103 ± 29 U. Carr) was found in peak lactation. The OSI developed similarly, with the highest value of 5.58 ± 0.94 in the calving and maternity period in the 1st lactation cows and the lowest value of 4.05 ± 1.21 in peak lactation and significant differences were found in many cases. Based on the results, the measurement of dROMs and OSI may be suitable for detecting oxidative stress in different lactation stages.