Surface biocompatibility of porous titanium structures with stem cells

Anotace: Successful tissue regeneration requires scaffolds with mechanical stability or biodegradability, surface roughness, and porosity to provide a suitable microenvironment for sufficient cell interaction, migration, cell proliferation, and differentiation. This study features the design, fabrication, and biocompatibility testing of Ti-6Al-4V titanium alloy scaffolds. Cylindrical titanium samples were tested, where each sample had a porous structure with pore sizes of 0.4 mm, 0.8 mm, and 1.0 mm respectively, which were seeded with chorionic-derived mesenchymal stem cells (CMSCs). The viability of the seeded CMSCs was evaluated using the MTT test. The aim of the study was to evaluate the cytotoxic effect and biocompatibility of porous titanium scaffolds. CMSCs showed the highest viability, adhesion to surfaces, and good proliferation on samples with 0.4 mm pore size, on the other hand, the pore size of 1.0 mm showed relatively lowest compatibility with cells and their proliferation. However, the viability of cells on all tested sizes of porous titanium scaffolds showed sufficient viability for future use in regenerative medicine.