Protective effect of celastrus paniculatus seeds extract against lipopolysaccharide-induced paw edema in rats

Celastrus paniculatus seeds were extracted with methanol by cold maceration for 7 days. The obtained dark brown coloured extract was evaluated for preliminary phytochemical screening and subjected for HPTLC analysis. Then the extract was evaluated for its action against LPS-induced paw edema in rats at the dose of 100, 200 and 400 mg/kg body weight by measuring the paw edema, nociceptive bioassays and estimating 5-HETE in serum. In addition, CPME was evaluated for in-vitro sEH inhibition activity.

Administration of CPME attenuates LPS-induced paw edema by 22.85% and serum 5-HETE levels by 9.56% (P<0.001) and elevated pain threshold by 112.19% (P<0.001). The CPME inhibits human, mouse and rat sEH activity with IC50 of 4.60 µg/mL, 4.17 µg/mL and 5.18 µg/mL, respectively. The present study results indicate that CPME has protective effect against LPS-induced inflammation and pain in rats. The possible mode of action is attenuation of 5-HETE levels and sEH inhibition. However, further research is warranted to elucidate and isolate the active constituents from Celastrus paniculatus seeds.