Inhibition of early stages of salmonella typhimurium biofilms by extracellular dna (edna) and genomic dna (gdna)

spectrophotometric measurements using PicoGreen® dye, it was determined that planktonic cells contain less eDNA compared to their
biofilm counterparts. As a result of incubation at 20 °C for 24h, eDNA contents of planktonic Salmonella Typhimurium SL1344 and
DMC4 cells were determined as 206 ng/ml and 211 ng/ml, respectively. However, eDNA amounts increases to 313.18 ng/ml for
SL1344 and 319.37 ng/ml for DMC4 at biofilm structure of the same strains. The effects of extracellular DNA (eDNA) released from
biofilm cells and genomic DNA (gDNA) isolated from Salmonella cells on the biofilm formation in the bacteria were investigated using
DNase I and Benzonase enzymes that digest DNA. In the experiments where both enzymes were applied, the active fragmentation of
eDNA and gDNA resulted in a statistically significant (p <0,01) decrease in biofilm formation at the end of 24 hours compared to nonenzyme treated samples, but this effect disappeared after 48 hours of incubation.