A liposomal controlled delivery system for plantago major l. Extracts and their biological activity for wound healing

Anotace: The objectives of this study are to determine the effective features of water and aqueous-ethanol extracts of Plantago major L. (Pm-dH2O and Pm-EtOH, respectively) in wound healing, such as antimicrobial, antioxidant potential, and fibroblast proliferation. Furthermore, it was targeted to obtain liposome structures loaded with Pm-extract for controlled release, characterize the obtained liposomes, and determine their release profiles. Antimicrobial activity (inhibition zone and minimum inhibition concentration), total antioxidant and oxidant (TAS, TOS, and OSI) potentials, total phenolic content (TPC), and total flavonoid content (TFC) of Pm extracts were tested. The radical scavenging activity of Pm extracts was quantified by the DPPH assay. Antioxidant capabilities of Pm extracts were determined with ABTS, CUPRAC, and FRAP. In vitro cytotoxicity tests of Pm extracts were examined on dermal fibroblast cells with MTT assay. Liposome structures loaded with Pm extracts were obtained using the ethanol injection method. Plant extract-loaded liposomes were characterized, and in vitro release kinetics were revealed by dialysis technique. Zobrazit více »