Anotace:
Vitex negundo Linn is a large aromatic shrub used as a traditional medicine for the treatment as antimicrobial, anticancer and diuretic properties belongs to the family Verbenaceae. In the present study an attempt had been made to study the antibacterial and hepatoprotective activity of V. negundo (VN) against LPS. The antibacterial activity of leaves, bark and seeds of VN were tested against different commonly occurring human pathogenic bacteria’s such as Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Klebsiella pneumonia. Among the different extracts and parts of plant, methanolic extract of leaves showed much more antibacterial activity against these different bacterial strains especially E.coli. VN was checked on HepG2 cells against the toxicity induced by LPS to prove the hepatoprotective activity. We showed that the VN induced ROS using JNK and MAPK pathways, decreased the apoptotic gene expressions such as COX2, IL 1 β, NFκβ and iNOS in HepG2 cells to protect the liver cells against LPS toxicity.