Expression, purification, and characterization of recombinant v, the newcastle disease virus non-structural protein

the vaccinated and naturally infected birds. If we could find a key marker that could differentiate, we would bring a great achievement to
the industry. NDV genome contains genes for six major structural proteins and two non-structural proteins. The non-structural protein V
could be a useful antigen for differentiating chickens infected with NDV from those vaccinated with inactivated whole virus.
We have constructed a recombinant plasmid using the C-terminal region of the non-structural V protein. Another recombinant plasmid
using the C-terminal polypeptide of structural P protein was developed as a control. We succeeded in expressing the unique C-terminal
part of the V protein in E. coli as a fusion protein with GST (GST-V), 40.8 kDa, as well as the C-terminal part of the P protein (GST-P),
55.4 kDa. Purified NDV-V and NDV-P proteins had sizes of 14.8 kDa and 29.4 kDa, respectively. The antigenicity of recombinant V
protein was confirmed with Western blotting assay, which makes the using of this protein as a biological marker prospective.