Optimization of the culture medium composition to increase the biosynthesis of recombinant human interleukin-7 in escherichia coli

quantitative and qualitative composition of the culture medium. As a result of optimization of the quantitative composition of organic
and mineral components of the culture medium using the matrix of planning a fractional factor experiment, it was possible to increase
the synthesis of rIL-7 by 1.3 times. It was proved that the use of supplements to the culture medium in the form of extracts of Clivia
miniata and Zephyranthes grandiflora (0.5-1.0%) stimulated the rIL-7 synthesis in recombinant bacterial cells in 1.3-1.4 times. The use
of phytoextracts at a concentration of 5.0% and more caused an inhibitory effect on the producer biosynthetic properties. It has been
established that the modulation of the biosynthetic activity of recombinant bacteria is due to the interaction of the biologically active
substances of plant extracts with the surface structures of the bacterial cell, namely, porins (OmpC and OmpF proteins). The stimulating
effect of vitamin K1 (15-25 mg per 1 ml of culture medium) in the liposomal form was proved; such approach allowed increasing the
synthesis of rIL-7 in 1.34-1.39 times. And the use of liposomal form of plant extracts as an approach to increasing the bioavailability of
biologically active substances is not appropriate.